| Title |
Detection of K‐ras gene mutation by liquid biopsy in patients with pancreatic cancer
|
|---|---|
| Published in |
Cancer (0008543X), March 2015
|
| DOI | 10.1002/cncr.29364 |
| Pubmed ID | |
| Authors |
Hideaki Kinugasa, Kazuhiro Nouso, Koji Miyahara, Yuki Morimoto, Chihiro Dohi, Koichiro Tsutsumi, Hironari Kato, Takehiro Matsubara, Hiroyuki Okada, Kazuhide Yamamoto |
| Abstract |
Cell-free circulating tumor DNA (ctDNA) in serum has been considered to be a useful candidate for noninvasive cancer diagnosis. The current study was designed to estimate the clinical usefulness of genetic analysis for ctDNA by digital polymerase chain reaction in patients with pancreatic cancer. The authors compared K-ras mutations detected in endoscopic ultrasound-guided fine-needle aspiration biopsy tissue DNA and in ctDNA from 75 patients with pancreatic cancer. K-ras mutations in the serum of 66 independent, consecutive patients with pancreatic cancer were also analyzed and the authors compared the results with survival rates. The frequencies of the mutations in tissue samples at G12V, G12D, and G12R in codon 12 were 28 of 75 samples (37.3%), 22 of 75 samples (29.3%), and 6 of 75 samples (8.0%), respectively. Conversely, the rates of the mutations in ctDNA were 26 of 75 samples (34.6%), 29 of 75 samples (38.6%), and 4 of 75 samples (5.3%), respectively. Overall, the K-ras mutation rates in tissue and ctDNA were 74.7% and 62.6%, respectively, and the concordance rate between them was 58 of 75 samples (77.3%). Survival did not appear to differ by the presence of K-ras mutations in tissue DNA, but the survival of patients with K-ras mutations in ctDNA was significantly shorter than that of patients without mutations in both a development set (P = .006) and an independent validation set (P = .002). The difference was especially evident in cases with a G12V mutation. Analysis of ctDNA is a new useful procedure for detecting mutations in patients with pancreatic cancer. This noninvasive method may have great potential as a new strategy for the diagnosis of pancreatic cancer as well as for predicting survival. Cancer 2015. © 2015 American Cancer Society. |
Mendeley readers
The data shown below were compiled from readership statistics for 215 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Ukraine | 1 | <1% |
| Germany | 1 | <1% |
| Ireland | 1 | <1% |
| Unknown | 212 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 40 | 19% |
| Student > Master | 28 | 13% |
| Researcher | 27 | 13% |
| Student > Bachelor | 19 | 9% |
| Student > Doctoral Student | 15 | 7% |
| Other | 38 | 18% |
| Unknown | 48 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 60 | 28% |
| Biochemistry, Genetics and Molecular Biology | 45 | 21% |
| Agricultural and Biological Sciences | 33 | 15% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 2% |
| Engineering | 5 | 2% |
| Other | 16 | 7% |
| Unknown | 51 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 April 2015.
All research outputs
#6,557,283
of 25,374,647 outputs
Outputs from Cancer (0008543X)
#5,383
of 14,096 outputs
Outputs of similar age
#71,811
of 278,145 outputs
Outputs of similar age from Cancer (0008543X)
#53
of 126 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 14,096 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.4. This one has gotten more attention than average, scoring higher than 61% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 278,145 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 126 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.