Title |
The Japanese Society of Pathology Guidelines on the handling of pathological tissue samples for genomic research: Standard operating procedures based on empirical analyses
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Published in |
Pathology International, February 2018
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DOI | 10.1111/pin.12631 |
Pubmed ID | |
Authors |
Yae Kanai, Hiroshi Nishihara, Yohei Miyagi, Tatsuhiro Tsuruyama, Kenichi Taguchi, Hiroto Katoh, Tomoyo Takeuchi, Masahiro Gotoh, Junko Kuramoto, Eri Arai, Hidenori Ojima, Ayako Shibuya, Teruhiko Yoshida, Toshiaki Akahane, Rika Kasajima, Kei‐ichi Morita, Johji Inazawa, Takeshi Sasaki, Masashi Fukayama, Yoshinao Oda |
Abstract |
Genome research using appropriately collected pathological tissue samples is expected to yield breakthroughs in the development of biomarkers and identification of therapeutic targets for diseases such as cancers. In this connection, the Japanese Society of Pathology (JSP) has developed "The JSP Guidelines on the Handling of Pathological Tissue Samples for Genomic Research" based on an abundance of data from empirical analyses of tissue samples collected and stored under various conditions. Tissue samples should be collected from appropriate sites within surgically resected specimens, without disturbing the features on which pathological diagnosis is based, while avoiding bleeding or necrotic foci. They should be collected as soon as possible after resection: at the latest within about 3 h of storage at 4°C. Preferably, snap-frozen samples should be stored in liquid nitrogen (about -180°C) until use. When intending to use genomic DNA extracted from formalin-fixed paraffin-embedded tissue, 10% neutral buffered formalin should be used. Insufficient fixation and overfixation must both be avoided. We hope that pathologists, clinicians, clinical laboratory technicians and biobank operators will come to master the handling of pathological tissue samples based on the standard operating procedures in these Guidelines to yield results that will assist in the realization of genomic medicine. |
X Demographics
Geographical breakdown
Country | Count | As % |
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India | 1 | 20% |
Spain | 1 | 20% |
Unknown | 3 | 60% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 4 | 80% |
Members of the public | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 34 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 8 | 24% |
Student > Master | 4 | 12% |
Lecturer | 3 | 9% |
Student > Bachelor | 3 | 9% |
Other | 2 | 6% |
Other | 4 | 12% |
Unknown | 10 | 29% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 12 | 35% |
Biochemistry, Genetics and Molecular Biology | 4 | 12% |
Agricultural and Biological Sciences | 2 | 6% |
Environmental Science | 1 | 3% |
Nursing and Health Professions | 1 | 3% |
Other | 4 | 12% |
Unknown | 10 | 29% |